CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue.

Authors:
Ulf Helwig, Paolo Gionchetti, Fernando Rizzello, Karen Lammers, Tanja Kühbacher, Stefan Schreiber, Marco Baggiolini, Mariagrazia Uguccioni, Massimo Campieri
Year of publication:
2004
Volume:
19
Issue:
2
Issn:
0179-1958
Journal title abbreviated:
INT J COLORECTAL DIS
Journal title long:
International journal of colorectal disease
Impact factor:
2.383
Abstract: 
These data support the importance of chemokines for the leukocyte recruitment in pouch tissue during acute pouchitis.Expression of IL-8, MCP-1, MCP-3, and IP-10 was significantly higher in pouchitis than normal pouch. The expression of MCP-1, MCP-3 and IP-10 were significantly lower after antibiotic treatment.Biopsy specimens postoperatively from ulcerative colitis patients with IPAA were obtained by endoscopy. Biopsies were taken from 8 patients with noninflamed IPAA and from 14 patients with an episode of acute pouchitis, before and after antibiotic treatment. Biopsies were stained for CD68, CD3, elastase, eotaxin, IP-10, MCP-1, MCP-3, and IL-8 and analyzed by NIH Image analyzer.Pouchitis is the major long-term complication after ileal pouch-anal anastomosis (IPAA) in patients operated on for ulcerative colitis. The cause is unknown, but both the history of ulcerative colitis and increased bacterial concentration are important factors. Chemokines are mediators for the recruitment of inflammatory cells to the site of inflammation. This study examined the tissue expression of a panel of specific chemokines and the corresponding recruitment of inflammatory cells in IPAA tissue with and without inflammation and after antibiotic treatment.