Immunochip analyses identify a novel risk locus for primary biliary cirrhosis at 13q14, multiple independent associations at four established risk loci and epistasis between 1p31 and 7q32 risk variants.

Authors:
Brian D Juran, Gideon M Hirschfield, Pietro Invernizzi, Elizabeth J Atkinson, Yafang Li, Gang Xie, Roman Kosoy, Michael Ransom, Ye Sun, Ilaria Bianchi, Erik M Schlicht, Ana Lleo, Catalina Coltescu, Francesca Bernuzzi, Mauro Podda, Craig Lammert, Russell Shigeta, Landon L Chan, Tobias Balschun, Maurizio Marconi, Daniele Cusi, E Jenny Heathcote, Andrew L Mason, Robert P Myers, Piotr Milkiewicz, Joseph A Odin, Velimir A Luketic, Bruce R Bacon, Henry C Bodenheimer, Valentina Liakina, Catherine Vincent, Cynthia Levy, Andre Franke, Peter K Gregersen, Fabrizio Bossa, M Eric Gershwin, Mariza deAndrade, Christopher I Amos, - -, Konstantinos N Lazaridis, Michael F Seldin, Katherine A Siminovitch
Year of publication:
2012
Volume:
21
Issue:
23
Issn:
0964-6906
Journal title abbreviated:
HUM MOL GENET
Journal title long:
Human molecular genetics
Impact factor:
5.985
Abstract: 
To further characterize the genetic basis of primary biliary cirrhosis (PBC), we genotyped 2426 PBC patients and 5731 unaffected controls from three independent cohorts using a single nucleotide polymorphism (SNP) array (Immunochip) enriched for autoimmune disease risk loci. Meta-analysis of the genotype data sets identified a novel disease-associated locus near the TNFSF11 gene at 13q14, provided evidence for association at six additional immune-related loci not previously implicated in PBC and confirmed associations at 19 of 22 established risk loci. Results of conditional analyses also provided evidence for multiple independent association signals at four risk loci, with haplotype analyses suggesting independent SNP effects at the 2q32 and 16p13 loci, but complex haplotype driven effects at the 3q25 and 6p21 loci. By imputing classical HLA alleles from this data set, four class II alleles independently contributing to the association signal from this region were identified. Imputation of genotypes at the non-HLA loci also provided additional associations, but none with stronger effects than the genotyped variants. An epistatic interaction between the IL12RB2 risk locus at 1p31and the IRF5 risk locus at 7q32 was also identified and suggests a complementary effect of these loci in predisposing to disease. These data expand the repertoire of genes with potential roles in PBC pathogenesis that need to be explored by follow-up biological studies.