Transcript Quantification

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Scientists

Facts & Details: 

A.   Transcript quantification, real time PCR based, multiple targets

Technology / platform:

Life Technologies / Applied Biosystems TaqMan, Microfluidic Cards

Setup options:       

48 target transcripts, 8-plex

 

96 target transcripts, 4-plex

 

384 target transcripts, no multiplex

Sample requirements:     

35 ng quality controlled total RNA

Estimated costs:         

100-200 € / sample, depending on setup applied

Estimated timeline:  

depending on queue; processing wetlab: 2 weeks; analysis: 1 week


 

B.   Transcript quantification, real time PCR based, single targets

Technology / platform:

Life Technologies / Applied Biosystems TaqMan, tubed assays

Setup options:       

1 target gene & at least one housekeeping gene

Sample requirements:     

100 ng quality controlled total RNA

Estimated costs:         

3-200 €/sample, depending on multiplex applied

Estimated timeline:  

depending on queue; processing wetlab: 2 weeks; analysis: 1 week