A comprehensive, cell specific microRNA catalogue of human peripheral blood

August 31, 2017 - 14:45

With the study published in Nucleic Acids Research this month, the IKMB together with colleagues from Germany and Lithuania provides the largest comprehensive reference dataset of detailed miRNA expression profiles from seven types of human peripheral blood cells (NK cells, B lymphocytes, cytotoxic T lymphocytes, T helper cells, monocytes, neutrophils and erythrocytes), serum, exosomes and whole blood. A total of 162 blood samples from healthy volunteers were included in this study, a subset of which were typed and sorted using fluorescence activated cell sorting and magnetic activated cell sorting. The overall dataset was generated from 450 small RNA libraries using high-throughput sequencing.

The authors have, for the first time, shown that 3′ trimming modifications as well as composition of 3′ added non-templated nucleotides are distributed in a lineage-specific manner – the closer the hematopoietic progenitors are, the higher their similarities in sequence variation of the 3′ end. Furthermore, they have defined the blood cell-specific miRNA and isomiR expression patterns and identified novel cell type specific miRNA candidates. The study provides the most comprehensive miRNA catalogue of human peripheral blood, which can be used as a reference for future studies. The dataset is publicly available on GEO and also can be explored interactively following this link.

This catalogue was set up of scientists from Germany and Lithuania. First author is a doctoral researcher, Simonas Juzenas who is doing his doctoral project at the Institute of Clinical Molecular Biology IKMB, Kiel University Germany and at the Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania.

More information about the authorship:
Simonas Juzenas1,2Geetha Venkatesh1Matthias Hübenthal1Marc P. Hoeppner1, Zhipei Gracie Du1Maren Paulsen1Philip Rosenstiel1, Philipp Senger3, Martin Hofmann-Apitius3, Andreas Keller4, Limas Kupcinskas2 Andre Franke1Georg Hemmrich-Stanisak1

Institute of Clinical Molecular Biology IKMB, Kiel University German
Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania 
 Department of Bioinformatics, Fraunhofer Institute for Algorithms and Scientific Computing (SCAI), Sankt Augustin, Germany 
Clinical Bioinformatics, Saarland University, 66125 Saarbrücken, Germany

Full text of the publication: Nucleic Acids Research, Aug 11, 2017

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