Exome sequencing allows for targeted sequencing of all coding regions of the human genome while needing only a fraction of the sequencing of a whole genome. In addition, gene panels for different applications are available and several providers offer custom gene panels that can be sequenced at the IKMB. Depending on the sequencing platform, exomes are mostly processed in pools of 6 samples. If you submit fewer samples, we will try to pool them with others, but this may then take longer.

Supported sequencers

• NovaSeq 6000
• HiSeq 3000 & 4000
• HiSeq 2500
• NextSeq 500
• MiSeq (for small targets from custom enrichment panels only)

Supported protocols

•  Nextera Flex for Enrichtment with IDT xGen Exome
• custom enrichment panels after prior consultation

Sample specifications for all supported protocols

You can download the sequencing request for whole exome sequencing and custom enrichment here.

Please send the filled sequencing request to s.franzenburg [at] ikmb.uni-kiel.de.