Atopic Dermatitis Is an IL-13-Dominant Disease with Greater Molecular Heterogeneity Compared to Psoriasis.

Authors:
Lam C Tsoi, Elke Rodriguez, Frauke Degenhardt, Hansjörg Baurecht, Ulrike Wehkamp, Natalie Volks, Silke Szymczak, William R Swindell, Mrinal K Sarkar, Kalpana Raja, Shuai Shao, Matthew Patrick, Yilin Gao, Ranjitha Uppala, Bethany E Perez White, Spiro Getsios, Paul W Harms, Emanual Maverakis, James T Elder, Andre Franke, Johann E Gudjonsson, Stephan Weidinger
Year of publication:
2019
Volume:
-
Issue:
-
Issn:
0022-202X
Journal title abbreviated:
J INVEST DERMATOL
Journal title long:
Journal of investigative dermatology
Impact factor:
7.143
Abstract:
Atopic dermatitis (AD) affects up to 20% of children and adults worldwide. To gain a deeper understanding of the pathophysiology of AD, we conducted a large-scale transcriptomic study of AD with deeply sequenced RNA-sequencing samples using long (126-bp) paired-end reads. In addition to the comparisons against previous transcriptomic studies, we conducted in-depth analysis to obtain a high-resolution view of the global architecture of the AD transcriptome and contrasted it with that of psoriasis from the same cohort. By using 147 RNA samples in total, we found striking correlation between dysregulated genes in lesional psoriasis and lesional AD skin with 81% of AD dysregulated genes being shared with psoriasis. However, we described disease-specific molecular and cellular features, with AD skin showing dominance of IL-13 pathways, but with near undetectable IL-4 expression. We also demonstrated greater disease heterogeneity and larger proportion of dysregulated long noncoding RNAs in AD, and illustrated the translational impact, including skin-type classification and drug-target prediction. This study is by far the largest study comparing the AD and psoriasis transcriptomes using RNA sequencing and demonstrating the shared inflammatory components, as well as specific discordant cytokine signatures of these two skin diseases.