miRNome Profiling and Functional Analysis Reveal Involvement of hsa-miR-1246 in Colon Adenoma-Carcinoma Transition by Targeting <i>AXIN2</i> and <i>CFTR</i>.

Authors:
Rokas Lukosevicius, Simonas Juzenas, Violeta Salteniene, Ugne Kulokiene, Justina Arstikyte, Georg Hemmrich-Stanisak, Andre Franke, Alexander Link, Paulius Ruzgys, Saulius Satkauskas, Henrikas Pauzas, Tadas Latkauskas, Gediminas Kiudelis, Francesc Balaguer, Juozas Kupcinskas, Jurgita Skieceviciene
Year of publication:
2022
Volume:
23
Issue:
4
Issn:
1422-0067
Journal title abbreviated:
INT J MOL SCI
Journal title long:
International journal of molecular sciences
Impact factor:
6.208
Abstract:
Regulatory changes occurring early in colorectal cancer development remain poorly investigated. Since the majority of cases develop from polyps in the adenoma-carcinoma transition, a search of early molecular features, such as aberrations in miRNA expression occurring prior to cancer development, would enable identification of potentially causal, rather than consequential, candidates in the progression of polyp to cancer. In the current study, by employing small RNA-seq profiling of colon biopsy samples, we described differentially expressed miRNAs and their isoforms in the adenoma-carcinoma transition. Analysis of healthy-adenoma-carcinoma sequence in an independent validation group enabled us to identify early deregulated miRNAs including hsa-miR-1246 and hsa-miR-215-5p, the expressions of which are, respectively, gradually increasing and decreasing. Loss-of-function experiments revealed that inhibition of hsa-miR-1246 lead to reduced cell viability, colony formation, and migration rate, thereby indicating an oncogenic effect of this miRNA in vitro. Subsequent western blot and luciferase reporter assay provided evidence of hsa-miR-1246 being involved in the regulation of target <i>AXIN2</i> and <i>CFTR</i> genes' expression. To conclude, the present study revealed possible involvement of hsa-miR-1246 in early colorectal cancer development and regulation of tumor suppressors AXIN2 and CFTR.