Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome.

Authors:
A Bihimaier, U Römling, T F Meyer, B Tümmler, C P Gibbs
Year of publication:
1991
Volume:
5
Issue:
10
Issn:
0950-382X
Journal title abbreviated:
Mol. Microbiol.
Journal title long:
Molecular microbiology
Impact factor:
3.979
Abstract:
A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.