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Scientists
- Genome wide transcriptome analysis, sequencing based
Technology / platform: |
Illumina HiSeq and others |
Setup options: |
full transcriptome, coverage depending on technology applied multiplexing up to 96 samples |
Sample requirements: |
100-500 ng quality controlled total RNA |
Estimated costs: |
300-600 €/sample, depending on technology applied / multiplexing |
Estimated timeline: |
depending on queue; processing wetlab: 4 weeks; analysis: 6 weeks |
2. Genome wide transcriptome analysis, array based
Technology / platform: |
Affymetrix GeneChip |
Setup options: |
Human Gene 2.0 st (2013: 45 000 transcripts) |
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Human Gene 1.0 st (2010: 29 000 transcripts) |
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HG-U133 Plus 2.0 (2004: 55 000 transcripts) |
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miRNA 2.0 (2012: 20 000 transcripts) |
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miRNA 1.0 (2009: 08 000 transcripts) |
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Mouse Gene 2.0 st (2013: 34 000 transcripts) |
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Mouse Gene 1.0 st (2009: 29 000 transcripts) |
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Mouse 430 2.0 |
Sample requirements: |
100 ng quality controlled total RNA |
Estimated costs: |
397 €/sample |
Estimated timeline: |
depending on queue; processing wetlab: 2 weeks; analysis: 2 weeks |