Next generation sequencing based transcriptome analysis allows genome wide monitoring of gene expression. Most prominent analysis types are focusing on transcript levels, splicing events or allelic imbalance. Due to the dynamic nature of the transcriptome, the experimental setup plays a critical role. The level of transcriptome complexity for a given species determines the number of samples that can be pooled in one experimental run (lane).

Supported sequencers

• NovaSeq 6000
• HiSeq 3000 & 4000

Supported protocols

• TruSeq stranded mRNA (poly-A)
• TruSeq stranded total RNA (Ribo-Zero Gold)
• TruSeq RNA Exome
• Chromium scRNAseq

Sample specifications for all supported protocols

You can download the sequencing request for transcriptome sequencing here.

Please send the filled sequencing request to s.franzenburg [at] ikmb.uni-kiel.de