Due to their abundance within the human genome, Single Nucleotide Polymorphisms (SNPs) have become the geneticist's tool of choice for association mapping. At the moment, public databases hold more than 44 million verified SNPs (dbSNP build 138) and this number keeps increasing due to ongoing large-scale genome resequencing projects.
The Institute of Clinical Molecular Biology (IKMB) operates a high-throughput genotyping core facility, which is one of the largest academic platforms of its kind. It is equipped with three different genotyping technologies to handle projects of all sizes and provides in-house capacity to replicate and technically validate interesting hits from screening studies.
A high grade of automation allows the handling of nearly all wet-lab steps on Tecan Evo robots based on standard operation procedures, which provides maximum accuracy and reproducibility. Genotypes are stored in a custom database and can be exported in standard analysis formats (e.g. for PLINK) through this system. The described genotyping platform is built on top of a physically separated high-throughput pre-PCR lab and all laboratory processes are monitored by the local laboratory information management system (LIMS).
The IKMB's genotyping platform offers services to academic partners.
Regular platform meeting:
Bi-weekly Wednesdays at 08:10 AM (odd weeks only) for DNA laboratory, Genotyping, Microbiome laboratory and Sanger, 8:30 AM (odd weeks only) for "Next-Generation" Sequencing
ZMB, conference room 4th floor, Am Botanischen Garten 11, 24118 Kiel
The meeting is open for collaborators who want to discuss or prioritize their ongoing projects at the IKMB. For registering a new project please contact the respective platform leader.